Joey D'Auria - Butch Cat, Jeff, Meathelda, TV Narrator, Dawn O' Day, Droopy, Conductor, Butch's Angel, Butch's Devil, Red Herring ( All Cat Jazz only).

Rachael MacFarlane - Hildie, Mummy, Bo Peep.

Rene Mujica - Newt, Bear, Tom's 7th Life.

Kath Soucie - Tuffy Mouse, Mimi, Aunt Claire, Cozette, Gigi, Doofy Mouse ( School of Hard Knocks only).

Stephen Stanton - Rick (Season 2 onwards), Muscles Mouse, Uncle Pecos, Jack Benny Mouse, Commercial voice, Frankie, Dixie Wolf, Junior, Sweet-Tooth Malone, Ed, Mack, Fifi's ex-boyfriend, Peterson's Cat, Country Wolf, City Wolf, Whispering Voice, Squirrel, Delivery Driver, Hunter, Cop, Neighbor, Reporter, Pizza Guy, Sergeant Jones, Mynah, Boss Dog, Barney Bear, Reginald Dane, Hoops McJumpalot, Papa, Benny.

Grey Delisle-Griffin - Ginger, Winston's Owner, Drone, Beatrice.

Rick Zieff - Spike Bulldog, Meathead Cat (Season 2 onwards), Barkley, Skid, Dutch, Santa Claus, Skullie, Claude, Trucket Man, Squirrel, Bertrand, Man 3, Tom's Lawyer, Bug, Peter, Mike Bulldog, Policeman, Preston LaPlume, Tom's Devil, Tom's Angel, Jerry's Devil, Jerry's Angel.

Frank Welker (Uncredited) - Tyke Bulldog.

Rich Danhakl (uncredited) – Tom Cat, Jerry Mouse.

Mel Blanc (Archive audio Uncredited) - Tom Cat, Jerry Mouse (laughter and screams).

Lang (Archive audio Uncredited) - Tom Cat

William Hanna (Archive audio Uncredited) - Tom Cat, Jerry Mouse (laughter and screams).

However beginning with Season 2, each episode had three 7-minute segments much like the original shorts. Initially Season 1 had two 11-minute segments in each episode.

(This scenario is first used beginning with Season 3)

Tom and Jerry living in a scary Transylvanian environment in a parody of Van Helsing.

Tom working as a butler in a Downton Abbey property.

(This scenario is retired in Seasons 2, 3, 4 and 5)

Jerry living in a lab with a rat companion named Napoleon, with Tom being an alley cat.

Tom and Jerry working together as detectives in Tolucaville (Los Angeles as of Season 2) by operating a detective agency called 'The Cat and Mouse Detectives', complete with a narrator.

(This scenario is retired in Seasons 3, 4 and 5)

Tom and Jerry being pets to two witches named Beatie and Hildie.

Tom and Jerry's typical antics in a modern-day urban setting, usually set in a house owned by Rick and Ginger.

Each episode of the series follows either one of these scenarios: This reboot of the Tom and Jerry franchise follows the antics of Tom in his pursuit to catch Jerry.

Tom and jerry episodes 2010